Department of Chemistry
Analysis of Circulating Tumor Cells Laboratory
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Μolecular Analyses
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Circulating tumor cells (CTCs)
» ER, PR, HER2, EGFR gene expression
Circulating tumor cells (CTCs)
CTCs enumeration (CellSearch System)
CTCs detection through CK-19 expression
CK-19, MAGE-A3, HER-2, TWIST1, hTERT α+β+, mammaglobin gene expression
ER, PR, HER2, EGFR gene expression
CD44, CD24, ALDH1, HPRT gene expression
Quantification of Androgen Receptor (AR) splice variants in CTCs
Detection of PIK3CA and ESR1 mutations
Detection of ESR1 methylation
Circulating tumor DNA (ctDNA)
ER, PR, HER2, EGFR gene expression
Molecular characterization of CTCs in peripheral blood of breast cancer patients
using multiplex RT-qPCR
Genes:
ER, PR, HER2, EGFR
Methodology:
multiplex RT-qPCR
Instrumentation:
LightCycler 2.0 (IVD, Roche Diagnostics)
Analytical characteristics:
exceptional analytical sensitivity and specificity
Requested sample:
20mL peripheral blood (EDTA) - pre-, during or post-therapy
Cost:
please contact Dr E.Lianidou (lianidou@chem.uoa.gr)